cd8α apc  (Miltenyi Biotec)

Journal: Microbiology Spectrum

Article Title: Amino acid insertion in the Meq protein of Marek’s disease virus, an avian oncogenic herpesvirus, accelerates tumorigenesis

doi: 10.1128/spectrum.03368-24

Figure Lengend Snippet: Gating strategy for the analysis of the dynamics of T-cell subsets involved in immune response in chickens infected with rMDVs. A representative gating strategy is shown for analyzing the dynamics of CD8α + γδ T cells, CD4 + γδ T cells, and CD8αα + T cells. Dead cells were excluded using Fixable Viability Dye eFluor780.

Article Snippet: The cells were stained with mouse anti-chicken CD3ε mAbs conjugated with PerCP-Cyanine5.5 dye, mouse anti-chicken CD4 mAbs conjugated with PE-Cy7, mouse anti-chicken CD8β mAbs conjugated with FITC, mouse anti-chicken CD4 mAbs conjugated with PE, and mouse anti-chicken CD8α mAbs conjugated with APC (Southern Biotech) for 30 min at 4°C in the dark.

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: Amino acid insertion in the Meq protein of Marek’s disease virus, an avian oncogenic herpesvirus, accelerates tumorigenesis

doi: 10.1128/spectrum.03368-24

Figure Lengend Snippet: Dynamics of T-cell subsets involved in immune response in chickens infected with rMDVs. The dynamics of CD8α + γδ T cells and CD8αα + T cells from spleens of chickens infected with vRB-1B-Meq or vRB-1B-L-Meq at each time point during the experimental period (7–49 dpi: control group: n = 4, vRB-1B_Meq-infected group: n = 4, vRB-1B_L-Meq-infected group: n = 4, 56 dpi: control group: n = 4, vRB-1B_Meq-infected group: n = 7). ( A ) The percentages of CD8α + T cells in the γδ T cell population from spleens, ( B ) the percentage of CD8αα + cells in CD8α + T cell population from spleens, and ( C ) the percentage of CD4 + CD8α - T cells in CD4 + T cell population from spleens were analyzed. Error bars indicate standard deviations. * P < 0.05, Kruskal–Wallis test (7–35 dpi), Mann–Whitney U test (49–56 dpi).

Article Snippet: The cells were stained with mouse anti-chicken CD3ε mAbs conjugated with PerCP-Cyanine5.5 dye, mouse anti-chicken CD4 mAbs conjugated with PE-Cy7, mouse anti-chicken CD8β mAbs conjugated with FITC, mouse anti-chicken CD4 mAbs conjugated with PE, and mouse anti-chicken CD8α mAbs conjugated with APC (Southern Biotech) for 30 min at 4°C in the dark.

Techniques: Infection, Control, MANN-WHITNEY